Effect of poly-herbal formulation in experimentally induced diabetic nephropathy in rats


  • Nirmal Parmar Department of Pharmacology, Parul Institute of Pharmacy & Research, Parul University, Wadhodia, Vadodara- 391760, Gujarat, India.
  • G.S. Chakraborthy Department of Pharmacology, Parul Institute of Pharmacy & Research, Parul University, Wadhodia, Vadodara- 391760, Gujarat, India.
  • Anas Jamsa Department of Pharmacology, Parul Institute of Pharmacy & Research, Parul University, Wadhodia, Vadodara- 391760, Gujarat, India.


Diabetic renal damage, Nephropathy,, Proteinuria, Anti-oxidant, Serum creatinine, PHF (Polyherbal formulation).


Introduction: Chronic kidney failure among people with diabetes mellitus (DM) is a burgeoning health problem that affects up to 25% of patients with type 2 DM. Current pharmacological treatments for diabetic nephropathy (DN) does not stop the attainment of renal complications. The intention of the current study was to explore the role of a polyherbal formulation (PHF) in diabetic-induced nephropathy in experimental animals.

Objectives:The objectives of the present study were to evaluate hydroalcoholic extract of Withaniasomnifera(leaves), Juniperuscommunis(barries) and Tinesporacordifolia(stem)for nephroprotective effect in streptozotocin (STZ) and Nicotinamide (NA) induced diabetic nephropathy (DN) in rats.

Materials and Methods: Diabetic male and female Wistar rats were divided into five groups; namely, Normal control (NC), Disease control (DC), standard control (Metformine 70 mg/kg, p.o), T1 (Polyherbal formulation 100 mg/kg, p.o.) and T2 (Polyherbal formulation 200 mg/kg, p.o). Treatment was continued for 6 weeks. Blood glucose levels, serum creatinine levels, blood urea nitrogen (BUN) levels, per day urine output, urinary protein concentration and histopathology of kidney were determined at appropriate time points.

Results: Untreated animals developed severe hyperglycemia and major disturbance in renal function. Rats in standard and Polyherbal formulation (PHF) treated groups had significantly lower plasma glucose levels, exhibited nearly normal urine volumes indicating better glomerular filtration rate. They had lower urinary protein, serum creatinine and BUN levels compared to untreated animals.

Conclusion: Treatment with PHF showed beneficial effect on DN which may be due to the improvement of renal function parameters and hyperlipidemic and inflammatory mediators.


. Maheshwari RA, Balaraman R, Sen AK, Seth AK. Effect of coenzyme Q10 alone and its combination with metformin on streptozotocin and nicotinamide induced diabetic nephropathy in rats. Indian J Pharmacology. 2014 May; 46 (6):627-32.

. Khaki A, Fathiazad F. Diabetic nephropathy-using herbals in diabetic nephropathy prevention and treatment-the role of ginger (Zingiberoficinale) and onion (Alliumcepa) in diabetics' nephropathy. Compendium Essays on Alternative Therapy. 2012 Jan:206–32.

. Satirapoj B, Adler SG. Comprehensive approach to diabetic nephropathy. Kidney Research Clinical Practice. 2014 Sep;33(3):121-31.

. Kamble HV, Bodhankar SL. Trigonelline and sitagliptin attenuates nicotinamide-streptozotocin induced diabetic nephropathy in Wistar rats. IntionalJourmal of Pharmacy and Pharmaceutical Science. 2013 Jan; 5:583-89.

. Makni M, Sefi M, Fetoui H, Garouiel M, Gargouri NK, Boudawara T, et al. Flax and pumpkin seeds mixture ameliorates diabetic nephropathy in rats. Food and Chemical Toxicology. 2010 Aug-Sep;48:2407–12.

. Patel SS, Shah RS, Goyal RK. Antihyperglycemic, antihyperlipidemic and antioxidant effects of Dihar, a polyherbal ayurvedic formulation in streptozotocin induced diabetic rats. Indian Journal of Experimental Biology. 2009 Jul; 47(7):564–70.

. Saleem S, Muhammad G, Hussain M. A, Altaf M, Bukhari S. Withania somnifera L.: Insights into the phytochemical profile, therapeutic potential, clinical trials, and future prospective. Iranian Journal of Basic Medical Sciences. 2020 Dec; 23(12), 1501–1526.

. Verma S.K, Kumar A. Therpeutic uses of Withaniasomnifera (Ashwagandha) with a note on withanolides and its pharmacological actions. Asian Journal of Pharmaceutical and Clinical research. 2011 Jul;4, 1–4.

. Choudhary N, Siddiqui M, Khatoon S. Pharmacognostic evaluation of Tinosporacordifolia (Willd.) Miers and identification of biomarkers. Journal of Ayurvedic and Herbal Medicine. 2014 Jul; 1(1), 13-16.

. Upadhyay A. K, Kumar K, Kumar A, Mishra HS. Tinosporacordifolia (Willd.) Hook. f. and Thoms. (Guduchi) - validation of the Ayurvedic pharmacology through experimental and clinical studies. International Journal of Ayurveda Research. 2010 Apr-Jun; 1(2), 112–121.

. Gumral N, Kumbul DD, Aylak F, Saygin M, Savik E. JuniperuscommunisLinn oil decreases oxidative stress and increases antioxidant enzymes in the heart of rats administered a diet rich in cholesterol. Toxicology and Industrial Health. 2015 Jan; 31(1):85-91.

. Modnicki D, Łabędzka J. Estimation of the total phenolic compounds in juniper sprouts (Juniperuscommunis, Cupressaceae) from different places at the kujawsko-pomorskie province. Herba Polonica Journal. 2009 Jan; 55(3): 127-132.

. Tunón H, Olavsdotter C, Bohlin L. Evaluation of anti-inflammatory activity of some Swedish medicinal plants. Inhibition of prostaglandin biosynthesis and PAF-induced exocytosis. Journal of Ethnopharmacology. 1995 Oct; 48(2):61-76.

. Lee S. M, Bressler R. Prevention of diabetic nephropathy by diet control in the db/db mouse. American Diabetes Association, Diabetes Care. 1981 Jan; 30(2), 106–111.

. Chow F, Ozols E, Nikolic-Paterson D, Atkins RC, Tesch, GH. Macrophages in mouse type 2 diabetic nephropathy: correlation with diabetic state and renal injury. Kidney International. 2004 Jan; 65(1): 116-128.

. Kaminskas A, Mazeikiene A. Biochemistry Laboratory Manual. 1st ed. Vilnius: Vilnius University, Faculty of Medicine; 2012. p.9

. Siri FM, Malhotra A, Factor SM, Sonnenblick EH, Fein FS. Prolonged ejection duration helps to maintain pump performance of the renal-hypertensive-diabetic rat heart: correlations between isolated papillary muscle function and ventricular performance in situ. Cardiovascular Research. 1997 Apr;34(1):230-40.

. Kawahito S, Kitahata H, Oshita S. Problems associated with glucose toxicity: Role of hyperglycemia-induced oxidative stress. World Journal of Gastroenterology. 2009 Sep;15(33):4137.

. Hemamalini K, Vijusha M. Antidiabetic activity of Methanolic extracts of leaves of Anogeissusacuminate, Roxburghexcandolle and SolanumpubescensWilld by Alloxan induced model in Rats. Der Pharmacia Lettre. 2012;4(5): 1445-60.

. De Zeeuw D, Ramjit D, Zhang Z, Ribeiro AB, Kurokawa K, Lash JP. Renal risk and renoprotection among ethnic groups with type 2 diabetic nephropathy: a post hoc analysis of RENAAL. Kidney International. 2006 May;69(9):1675-82.

. Nakamura Y, Myers B. Charge selectivity of proteinuria in diabetic glomerulopathy. American Diabetes Association. 1988 Sep;37(9):1202-11.

. Ruggenenti P, Remuzzi G. Time to abandon microalbuminuria? Kidney International. 2006 Oct;70(7):1214-22.

. Gomes I, Porto M, Santos M, Campagnaro B, Pereira T, Meyrelles S, et al. Renoprotective, anti-oxidative and antiapoptotic effects of oral low-dose quercetin in the C57BL/6J model of diabetic nephropathy. Lipids Health and Disease. 2014 Dec; 13(1):184.

. Han DC, Isono M, Hoffman BB, Ziyadeh FN. High glucose stimulates proliferation and collagen type I synthesis in renal cortical fibroblasts mediation by autocrine activation of TGF-β. Journal of American Society of Nephrology. 1999 Sep;10(9):1891-9.

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Parmar, N., G.S. Chakraborthy, & Jamsa, A. (2022). Effect of poly-herbal formulation in experimentally induced diabetic nephropathy in rats. Parul University Journal of Health Sciences and Research, 1(1), 14–24. Retrieved from https://pujhsr.paruluniversity.ac.in/index.php/home/article/view/6



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