Effect of poly-herbal formulation in experimentally induced diabetic nephropathy in rats
Keywords:
Diabetic renal damage, Nephropathy,, Proteinuria, Anti-oxidant, Serum creatinine, PHF (Polyherbal formulation).Abstract
Introduction: Chronic kidney failure among people with diabetes mellitus (DM) is a burgeoning health problem that affects up to 25% of patients with type 2 DM. Current pharmacological treatments for diabetic nephropathy (DN) does not stop the attainment of renal complications. The intention of the current study was to explore the role of a polyherbal formulation (PHF) in diabetic-induced nephropathy in experimental animals.
Objectives:The objectives of the present study were to evaluate hydroalcoholic extract of Withaniasomnifera(leaves), Juniperuscommunis(barries) and Tinesporacordifolia(stem)for nephroprotective effect in streptozotocin (STZ) and Nicotinamide (NA) induced diabetic nephropathy (DN) in rats.
Materials and Methods: Diabetic male and female Wistar rats were divided into five groups; namely, Normal control (NC), Disease control (DC), standard control (Metformine 70 mg/kg, p.o), T1 (Polyherbal formulation 100 mg/kg, p.o.) and T2 (Polyherbal formulation 200 mg/kg, p.o). Treatment was continued for 6 weeks. Blood glucose levels, serum creatinine levels, blood urea nitrogen (BUN) levels, per day urine output, urinary protein concentration and histopathology of kidney were determined at appropriate time points.
Results: Untreated animals developed severe hyperglycemia and major disturbance in renal function. Rats in standard and Polyherbal formulation (PHF) treated groups had significantly lower plasma glucose levels, exhibited nearly normal urine volumes indicating better glomerular filtration rate. They had lower urinary protein, serum creatinine and BUN levels compared to untreated animals.
Conclusion: Treatment with PHF showed beneficial effect on DN which may be due to the improvement of renal function parameters and hyperlipidemic and inflammatory mediators.
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